Pesticides / Biocides

Quality Assurance System: Good Laboratory Practice (GLP)

Analytical methods

• Determination of content of active substance (SANCO/3030/99 rev. 4)
• Analytical method validation (SANCO/3030/99 rev. 4)
• Five batch analysis (SANCO/3030/99 rev. 4)

Physico-chemical tests

• Spectral data (MS, NMR, IR, UV/VIS)
• State of substance (20 °C and 101.3 kPa)
• Melting/freezing point (EU A.1/OECD 102)
• Boiling point (EU A.2/OECD 103)
• Relative density (EU A.3/OECD 109)
• Vapour pressure (EU A.4/OECD 104)
• Water solubility (EU A.6/OECD 105)
• Partition coefficient n-octanol/water (EU A.8/OECD 107 + OECD 117)
• Flash point (EU A.9)
• Surface tension (EU A.5/OECD 115)
• Explosive properties (EU A.14)
• Oxidizing properties (EU A.17)
• Solubility in organic solvents (CIPAC MT 181)
• Dissociation constant (OECD 112)
• Viscosity (EN ISO 3140) and other…

Toxicological studies

• Acute oral toxicity - fixed dose procedure (OECD 420)
• Acute oral toxicity - acute toxic class method (OECD 423)
• Acute toxicity (dermal; OECD 402)
• In vitro skin corrosion: human skin model test (OECD 431)
• In vitro skin irritation (OECD 439)
• Bovine corneal opacity and permeability test method for identifying ocular corrosives and severe irritants (OECD 437)
• Local lymph node assay (OECD 429)
• Guinea pig maximisation test (OECD 406)
• Repeated dose (28-days) toxicity (oral; OECD 407)
• Sub-chronic oral toxicity test (repeated dose 90-days oral toxicity study in rodents; OECD 408) 
• Chronic toxicity test (OECD 452) 
• Combined chronic toxicity/carcinogenicity studies (OECD 453) 
• Reverse mutation test using bacteria (OECD 471) 
• In vitro mammalian cell micronucleus test (OECD 487) 
• In vitro mammalian chromosome aberration test (OECD 473) 
• In vitro mammalian cell gene mutation test (OECD 476) 
• In vivo mammalian erythrocyte micronucleus test (OECD 474) 
•  Prenatal developmental toxicity study (OECD 414) 
• Two-generation reproduction toxicity test (OECD 416) 

Ecotoxicological studies

• Acute toxicity for fish (OECD 203) 
• Daphnia magna, acute immobilisation test (OECD 202) 
• Algal inhibition test (OECD 201) 
• Activated sludge respiration inhibition test (OECD 209) 
• Daphnia magna reproduction test (OECD 211) 

Fate and behaviour in the environment 

• Hydrolysis as a function of pH (EU C.7) 
• Estimation of the adsorption coefficient (KOC) on soil and on sewage sludge using HPLC (EU C.19/OECD 121)  
• Ready biodegradability - Closed bottle test (OECD 301D) 
• Biodegradation - Zahn-Wellens test (EU C.9/OECD 302B) 

and other...